Review



dithiothreitol dtt  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Thermo Fisher dithiothreitol dtt
    Dithiothreitol Dtt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dithiothreitol+dtt/Dithiothreitol%2C+%3E99%2E5%25%2C+Molecular+Biology+Grade%2C+Ultrapure/pm42263078-47-17-19
    Average 95 stars, based on 1 article reviews
    dithiothreitol dtt - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    other:

    Article Title: Isoform-specific cofactor recruitment through the intrinsically disordered N-terminus of p63 underlies differential transcriptional activities
    Article Snippet: The beads were washed four times with 1 ml of 50 mM ammonium bicarbonate buffer (pH = 8.0) (ABC) (Fluka, 09830).

    Concentration Assay:

    Article Title: Resistance of E2F4DN to p38 MAPK phosphorylation reduces genotoxic cell death in N2a neuron-like cells.
    Article Snippet: N2a NCs were washed with phosphate buffered saline (PBS) and total RNA was then extracted with Qiazol (Qiagen) following the instructions of the manufacturer. .. The concentration of RNA in each sample was then measured using NanoDrop One UV-Vis (ThermoFisher Scientific), and 5 μg of total RNA AR TIC LE IN PR ES S were used for the synthesis of the cDNA in a 20 μl reaction mix containing 4 μl of 5x reaction buffer (Invitrogen), 40 units of RNAsin (Promega), 250 ng/μl of random primers (Invitrogen), 10 mM dNTPs (Biotools), 10 mM dithiothreitol (DTT) (Invitrogen), and 200 units of SuperScript IV (Invitrogen), following the instructions of the reverse transcriptase manufacturer. qRT-PCR qRT-PCR was performed with the QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) using SYBR Green (Cultek) in 15 μl reaction volume containing 1 μl cDNA and the upstream and downstream oligonucleotides (see Supplementary Table 3) at 10 μM. .. Cultures were placed on ice, washed with ice-cold PBS, and incubated for 3 minutes with 200 μl of cold extraction buffer containing 20 mM Tris-HCl (Roche) pH 6.8, 1 mM EDTA (Merck), 1% Triton-X100 (Sigma), 0.5% sodium dodecyl sulfate (Sigma-Aldrich), 10 mM -mercaptoethanol (Sigma-Aldrich), and 1× cOmplete Mini, and EDTA-free, protease inhibitor cocktail (Roche).

    Reverse Transcription:

    Article Title: Resistance of E2F4DN to p38 MAPK phosphorylation reduces genotoxic cell death in N2a neuron-like cells.
    Article Snippet: N2a NCs were washed with phosphate buffered saline (PBS) and total RNA was then extracted with Qiazol (Qiagen) following the instructions of the manufacturer. .. The concentration of RNA in each sample was then measured using NanoDrop One UV-Vis (ThermoFisher Scientific), and 5 μg of total RNA AR TIC LE IN PR ES S were used for the synthesis of the cDNA in a 20 μl reaction mix containing 4 μl of 5x reaction buffer (Invitrogen), 40 units of RNAsin (Promega), 250 ng/μl of random primers (Invitrogen), 10 mM dNTPs (Biotools), 10 mM dithiothreitol (DTT) (Invitrogen), and 200 units of SuperScript IV (Invitrogen), following the instructions of the reverse transcriptase manufacturer. qRT-PCR qRT-PCR was performed with the QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) using SYBR Green (Cultek) in 15 μl reaction volume containing 1 μl cDNA and the upstream and downstream oligonucleotides (see Supplementary Table 3) at 10 μM. .. Cultures were placed on ice, washed with ice-cold PBS, and incubated for 3 minutes with 200 μl of cold extraction buffer containing 20 mM Tris-HCl (Roche) pH 6.8, 1 mM EDTA (Merck), 1% Triton-X100 (Sigma), 0.5% sodium dodecyl sulfate (Sigma-Aldrich), 10 mM -mercaptoethanol (Sigma-Aldrich), and 1× cOmplete Mini, and EDTA-free, protease inhibitor cocktail (Roche).

    Quantitative RT-PCR:

    Article Title: Resistance of E2F4DN to p38 MAPK phosphorylation reduces genotoxic cell death in N2a neuron-like cells.
    Article Snippet: N2a NCs were washed with phosphate buffered saline (PBS) and total RNA was then extracted with Qiazol (Qiagen) following the instructions of the manufacturer. .. The concentration of RNA in each sample was then measured using NanoDrop One UV-Vis (ThermoFisher Scientific), and 5 μg of total RNA AR TIC LE IN PR ES S were used for the synthesis of the cDNA in a 20 μl reaction mix containing 4 μl of 5x reaction buffer (Invitrogen), 40 units of RNAsin (Promega), 250 ng/μl of random primers (Invitrogen), 10 mM dNTPs (Biotools), 10 mM dithiothreitol (DTT) (Invitrogen), and 200 units of SuperScript IV (Invitrogen), following the instructions of the reverse transcriptase manufacturer. qRT-PCR qRT-PCR was performed with the QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) using SYBR Green (Cultek) in 15 μl reaction volume containing 1 μl cDNA and the upstream and downstream oligonucleotides (see Supplementary Table 3) at 10 μM. .. Cultures were placed on ice, washed with ice-cold PBS, and incubated for 3 minutes with 200 μl of cold extraction buffer containing 20 mM Tris-HCl (Roche) pH 6.8, 1 mM EDTA (Merck), 1% Triton-X100 (Sigma), 0.5% sodium dodecyl sulfate (Sigma-Aldrich), 10 mM -mercaptoethanol (Sigma-Aldrich), and 1× cOmplete Mini, and EDTA-free, protease inhibitor cocktail (Roche).

    Real-time Polymerase Chain Reaction:

    Article Title: Resistance of E2F4DN to p38 MAPK phosphorylation reduces genotoxic cell death in N2a neuron-like cells.
    Article Snippet: N2a NCs were washed with phosphate buffered saline (PBS) and total RNA was then extracted with Qiazol (Qiagen) following the instructions of the manufacturer. .. The concentration of RNA in each sample was then measured using NanoDrop One UV-Vis (ThermoFisher Scientific), and 5 μg of total RNA AR TIC LE IN PR ES S were used for the synthesis of the cDNA in a 20 μl reaction mix containing 4 μl of 5x reaction buffer (Invitrogen), 40 units of RNAsin (Promega), 250 ng/μl of random primers (Invitrogen), 10 mM dNTPs (Biotools), 10 mM dithiothreitol (DTT) (Invitrogen), and 200 units of SuperScript IV (Invitrogen), following the instructions of the reverse transcriptase manufacturer. qRT-PCR qRT-PCR was performed with the QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) using SYBR Green (Cultek) in 15 μl reaction volume containing 1 μl cDNA and the upstream and downstream oligonucleotides (see Supplementary Table 3) at 10 μM. .. Cultures were placed on ice, washed with ice-cold PBS, and incubated for 3 minutes with 200 μl of cold extraction buffer containing 20 mM Tris-HCl (Roche) pH 6.8, 1 mM EDTA (Merck), 1% Triton-X100 (Sigma), 0.5% sodium dodecyl sulfate (Sigma-Aldrich), 10 mM -mercaptoethanol (Sigma-Aldrich), and 1× cOmplete Mini, and EDTA-free, protease inhibitor cocktail (Roche).

    SYBR Green Assay:

    Article Title: Resistance of E2F4DN to p38 MAPK phosphorylation reduces genotoxic cell death in N2a neuron-like cells.
    Article Snippet: N2a NCs were washed with phosphate buffered saline (PBS) and total RNA was then extracted with Qiazol (Qiagen) following the instructions of the manufacturer. .. The concentration of RNA in each sample was then measured using NanoDrop One UV-Vis (ThermoFisher Scientific), and 5 μg of total RNA AR TIC LE IN PR ES S were used for the synthesis of the cDNA in a 20 μl reaction mix containing 4 μl of 5x reaction buffer (Invitrogen), 40 units of RNAsin (Promega), 250 ng/μl of random primers (Invitrogen), 10 mM dNTPs (Biotools), 10 mM dithiothreitol (DTT) (Invitrogen), and 200 units of SuperScript IV (Invitrogen), following the instructions of the reverse transcriptase manufacturer. qRT-PCR qRT-PCR was performed with the QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) using SYBR Green (Cultek) in 15 μl reaction volume containing 1 μl cDNA and the upstream and downstream oligonucleotides (see Supplementary Table 3) at 10 μM. .. Cultures were placed on ice, washed with ice-cold PBS, and incubated for 3 minutes with 200 μl of cold extraction buffer containing 20 mM Tris-HCl (Roche) pH 6.8, 1 mM EDTA (Merck), 1% Triton-X100 (Sigma), 0.5% sodium dodecyl sulfate (Sigma-Aldrich), 10 mM -mercaptoethanol (Sigma-Aldrich), and 1× cOmplete Mini, and EDTA-free, protease inhibitor cocktail (Roche).

    Western Blot:

    Article Title: Chemical Genetic Screen Identifies PSD3 as a Direct Substrate of NUAK1 that Regulates Dendritic Spine Maturation
    Article Snippet: The microscope was equipped with a Zeiss Stage Top incubation and gas system for Lattice SIM inverted microscopes, enabling temperature, humidity, and CO2 control during live imaging. .. Samples for Western blot analysis were treated with NuPAGE LDS sample buffer (4×) (Invitrogen) with Dithiothreitol (DTT) at 200mM and subsequently heated for 10 min at 95°C. .. Samples were electrophoresed on NuPAGE 4 to 12% Bis-Tris polyacrylamide gels (Invitrogen) with NuPAGE MOPS or MES running buffer (Invitrogen).

    Electrophoresis:

    Article Title: TNG961 is a selective oral HBS1L molecular glue degrader for the treatment of FOCAD-deleted cancers
    Article Snippet: Protein concentration was quantified using a BCA Protein Assay (Thermo Fisher Scientific 23227) or Pierce 660 nM Protein Assay (Thermo Fisher Scientific 22660). .. An equal volume of LDS sample buffer containing Dithiothreitol (DTT) (Thermo Fisher Scientific B0008/B0009) was added to the lysate and samples were heated at 95oC for 5–10 min. Proteins were resolved via electrophoresis on 4-12% Bis-Tris gels (Thermo Fisher Scientific WG1402BOX) (20-40 μg/lane) and transferred to nitrocellulose membranes (Thermo Fisher Scientific IB33002). .. Simple Western (Jess): Cell pellets were lysed in RIPA buffer (Thermo Fisher Scientific 89900) supplemented with protease inhibitors (Thermo Fisher Scientific 1861281) and universal nuclease (VWR PI88701).

    Isolation:

    Article Title: Mitochondrial translation elongation controls OXPHOS biogenesis by coordinating synthesis and folding of mitochondrially encoded proteins.
    Article Snippet: In brief Xie et al. show that mitochondrial translation elongation rate influences OXPHOS biogenesis.. In the filamentous fungus Neurospora crassa, MRM1 slows elongation speed independently of its methyltransferase activity by simultaneously binding mitoribosomes and mRNAs, thereby coordinating synthesis with folding and membrane insertion of mitochondrially encoded OXPHOS subunits.. MRM1 N-terminal

    Lysis:

    Article Title: Mitochondrial translation elongation controls OXPHOS biogenesis by coordinating synthesis and folding of mitochondrially encoded proteins.
    Article Snippet: In brief Xie et al. show that mitochondrial translation elongation rate influences OXPHOS biogenesis.. In the filamentous fungus Neurospora crassa, MRM1 slows elongation speed independently of its methyltransferase activity by simultaneously binding mitoribosomes and mRNAs, thereby coordinating synthesis with folding and membrane insertion of mitochondrially encoded OXPHOS subunits.. MRM1 N-terminal



    Similar Products

    96
    Gold Biotechnology Inc dithiothreitol
    Dithiothreitol, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dithiothreitol+dtt/DTT+(Dithiothreitol)+(%3E+99%25+pure)+Protease+free/pm42126937-93-90-92
    Average 96 stars, based on 1 article reviews
    dithiothreitol - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    95
    Thermo Fisher dithiothreitol dtt
    Dithiothreitol Dtt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dithiothreitol+dtt/Dithiothreitol%2C+%3E99%2E5%25%2C+Molecular+Biology+Grade%2C+Ultrapure/pm42263078-47-17-19
    Average 95 stars, based on 1 article reviews
    dithiothreitol dtt - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    96
    Cytiva Europe dithiothreitol dtt
    Dithiothreitol Dtt, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dithiothreitol+dtt/SOURCE+15Q/pm41850277-481-8-15
    Average 96 stars, based on 1 article reviews
    dithiothreitol dtt - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    MedChemExpress dithiothreitol dtt
    Dithiothreitol Dtt, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dithiothreitol+dtt/DL-Dithiothreitol/pmc13209901-157-2-12
    Average 96 stars, based on 1 article reviews
    dithiothreitol dtt - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    94
    MedChemExpress dtt
    Dtt, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dithiothreitol+dtt/L-Dithiothreitol/pm42380090-117-32-33
    Average 94 stars, based on 1 article reviews
    dtt - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    86
    Fisher Scientific i1149 dithiothreitol dtt fisher scientific cat
    I1149 Dithiothreitol Dtt Fisher Scientific Cat, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dithiothreitol+dtt/dithiothreitol+dtt/pm42284144-330-271-274
    Average 86 stars, based on 1 article reviews
    i1149 dithiothreitol dtt fisher scientific cat - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Sangon Biotech 1 4 dithiothreitol dtt
    1 4 Dithiothreitol Dtt, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dithiothreitol+dtt/dtt/pm42160835-69-41-43
    Average 86 stars, based on 1 article reviews
    1 4 dithiothreitol dtt - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    96
    Gold Biotechnology Inc dithiothreitol dtt
    Dithiothreitol Dtt, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dithiothreitol+dtt/DTT+(Dithiothreitol)+(%3E+99%25+pure)+Protease+free/bio_rxiv__64898__2026__05__07__723637-113-15-17
    Average 96 stars, based on 1 article reviews
    dithiothreitol dtt - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad laemmli loading buffer
    Laemmli Loading Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dithiothreitol+dtt/Dithiothreitol+(DTT)/pmc13109723-56-6-9
    Average 96 stars, based on 1 article reviews
    laemmli loading buffer - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results